Pérez-Tomás R, Culleré X, Díaz C
Departament de Biologia Cellular i Anatomia Patològica, Facultat de Medicina, Universitat de Barcelona, Spain.
Gastroenterology. 1993 Mar;104(3):789-95. doi: 10.1016/0016-5085(93)91014-9.
Transforming growth factor alpha (TGF-alpha) is a 50-amino acid polypeptide that has been related to cell proliferation and differentiation.
Proximal and distal colon from fetal, newborn, and adult rats were studied by immunohistochemical techniques using a monoclonal antibody against human and rat TGF-alpha.
Immunoreactive TGF-alpha (IR-TGF-alpha) first appeared in distal colon at 18 days of gestation when the proximal colon remained negative. At all ages studied, the staining for TGF-alpha at the base of the crypts in the distal colon showed a supranuclear pattern. At 22 days of gestation and until 9 days of postnatal development, the proximal colon is negative for TGF-alpha. From day 10 to 24 of postnatal development, IR TGF-alpha cells with a cytoplasmic staining were confined to the lower half of the villi. Afterwards, cells at the crypts showed supranuclear staining and cells in the surface epithelium a cytoplasmic reaction.
Age- and region-dependent expression of TGF-alpha in the rat colon suggests a functional role for TGF-alpha in the establishment and maintenance of proliferation and differentiation during development.
转化生长因子α（TGF-α）是一种由50个氨基酸组成的多肽，与细胞增殖和分化有关。
采用抗人及大鼠TGF-α单克隆抗体，通过免疫组织化学技术对胎鼠、新生大鼠及成年大鼠的近端和远端结肠进行研究。
免疫反应性TGF-α（IR-TGF-α）最早在妊娠18天时出现在远端结肠，此时近端结肠呈阴性。在所有研究年龄阶段，远端结肠隐窝底部的TGF-α染色呈核上模式。在妊娠22天直至出生后9天的发育阶段，近端结肠的TGF-α呈阴性。从出生后第10天到第24天，具有细胞质染色的IR TGF-α细胞局限于绒毛的下半部分。此后，隐窝处的细胞呈核上染色，表面上皮细胞呈细胞质反应。
大鼠结肠中TGF-α的表达随年龄和区域而变化，提示TGF-α在发育过程中对细胞增殖和分化的建立及维持具有功能性作用。
