Chanh T C, Romanowski M J, Hewetson J F
Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78228.
Immunol Invest. 1993 Feb;22(1):63-72. doi: 10.3109/08820139309066194.
A BALB/c murine IgG1 monoclonal antibody, designated BG11-G2, specific for ricin was generated. BG11-G2 antibody did not bind to either purified ricin chain A or chain B, but recognized an antigenic determinant whose conformation requires the combination of the two chains in the formation of the native ricin molecule. It did not react with the protein synthesis inhibitor, T-2 mycotoxin, or with the sodium channel blockers, saxitoxin and tetrodotoxin. As little as 0.78 micrograms/ml of BG11-G2 IgG1 anti-ricin monoclonal antibody completely protected against the in vitro toxicity of ricin as determined by [3H]leucine uptake in EL-4 cell assays. Passive intraperitoneal infusion of purified BG11-G2 antibody into BALB/c mice one day prior to a lethal challenge with ricin considerably delayed the onset of toxicity and death. Better protection was obtained with BG11-G2 infused before and after ricin challenge.
制备了一种针对蓖麻毒素的BALB/c小鼠IgG1单克隆抗体，命名为BG11-G2。BG11-G2抗体既不与纯化的蓖麻毒素A链或B链结合，而是识别一种抗原决定簇，其构象需要两条链在天然蓖麻毒素分子形成过程中结合。它不与蛋白质合成抑制剂T-2毒素反应，也不与钠通道阻滞剂石房蛤毒素和河豚毒素反应。在EL-4细胞试验中，通过[3H]亮氨酸摄取测定，低至0.78微克/毫升的BG11-G2 IgG1抗蓖麻毒素单克隆抗体就能完全保护细胞免受蓖麻毒素的体外毒性。在用蓖麻毒素进行致死性攻击前一天，将纯化的BG11-G2抗体被动腹腔内注入BALB/c小鼠，可显著延迟毒性发作和死亡时间。在蓖麻毒素攻击前后注入BG11-G2可获得更好的保护效果。
