Kowalewich N, Hawkins E C, Skowronek A J, Clemo F A
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907.
J Am Vet Med Assoc. 1993 Feb 1;202(3):423-6.
Histoplasma capsulatum organisms were identified by cytologic evaluation in the thoracic and abdominal effusions of a 5-year-old sexually intact male Cocker Spaniel that was referred because of anorexia and lethargy. Treatment with amphotericin B and ketoconazole was instituted. The dog developed respiratory arrest, a complication of the disseminated disease, and died. Necropsy findings included pleural effusion, hepatomegaly, and enlarged tracheobronchial, hilar, mediastinal, and mesenteric lymph nodes. Granulomas containing periodic acid-Schiff (PAS)-positive yeast-like organisms identified as H capsulatum were seen in the lungs, liver, and lymph nodes. The lymphatic vessels were dilated, and fibrosis of the portal and periportal regions of the liver was noticed. Identification of Histoplasma organisms by cytologic examination of pleural and abdominal effusions is a rare laboratory finding and can provide a minimally invasive and inexpensive definitive diagnosis of histoplasmosis.
荚膜组织胞浆菌是在一只5岁性成熟雄性可卡犬的胸腔和腹腔积液中通过细胞学评估鉴定出来的，该犬因厌食和嗜睡前来就诊。开始用两性霉素B和酮康唑进行治疗。这只狗出现了呼吸骤停，这是播散性疾病的一种并发症，最终死亡。尸检结果包括胸腔积液、肝肿大以及气管支气管、肺门、纵隔和肠系膜淋巴结肿大。在肺、肝和淋巴结中可见含有经碘酸希夫（PAS）染色呈阳性的酵母样生物体（鉴定为荚膜组织胞浆菌）的肉芽肿。淋巴管扩张，肝脏门静脉和门静脉周围区域出现纤维化。通过对胸腔和腹腔积液进行细胞学检查来鉴定组织胞浆菌是一种罕见的实验室发现，可为组织胞浆菌病提供微创且廉价的确诊诊断。
