胰岛素样生长因子II和转甲状腺素蛋白基因在发育中的大鼠脉络丛中的差异表达。

Differential expression of the insulin-like growth factor II and transthyretin genes in the developing rat choroid plexus.

作者信息

Cavallaro T, Martone R L, Stylianopoulou F, Herbert J

机构信息

Department of Neurology, Columbia University, College of Physicians and Surgeons, New York, NY.

出版信息

J Neuropathol Exp Neurol. 1993 Mar;52(2):153-62. doi: 10.1097/00005072-199303000-00008.

DOI:10.1097/00005072-199303000-00008

PMID:8440997

Abstract

Choroid plexus (CP) development may depend on an inductive interaction between primordial CP epithelium and the overlying mesenchyme. Expression of the two CP epithelial-expressed genes, transthyretin (TTR) and insulin-like growth factor II (IGF-II), were studied by in situ hybridization in the developing rat. Transthyretin mRNA is expressed in abundance in the primordial CP epithelium prior to CP morphogenesis (e10-11) but IGF-II mRNA expression begins later (e13) and increases gradually as morphogenesis proceeds. In the CP stroma (mesenchyme), IGF-II mRNA is abundant prior to CP morphogenesis but decreases as embryogenesis proceeds and is absent in the adult. Our findings suggest that IGF-II may play an early paracrine and later autocrine role in CP development. A model is proposed in which IGF-II synthesized by mesenchyme serves as an inducing principle for CP epithelial differentiation.

摘要

脉络丛（CP）的发育可能依赖于原始CP上皮与覆盖其上的间充质之间的诱导性相互作用。通过原位杂交技术，在发育中的大鼠体内研究了两种CP上皮表达基因，即转甲状腺素蛋白（TTR）和胰岛素样生长因子II（IGF-II）的表达情况。在CP形态发生之前（胚胎第10 - 11天），原始CP上皮中大量表达转甲状腺素蛋白mRNA，但IGF-II mRNA的表达开始较晚（胚胎第13天），并随着形态发生的进行而逐渐增加。在CP基质（间充质）中，IGF-II mRNA在CP形态发生之前大量存在，但随着胚胎发育的进行而减少，在成年个体中则不存在。我们的研究结果表明，IGF-II可能在CP发育中发挥早期旁分泌和后期自分泌作用。提出了一个模型，其中间充质合成的IGF-II作为CP上皮分化的诱导因子。