Wieczorek Z, Siemion I Z, Zimecki M, Bolewska-Pedyczak E, Wieland T
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Peptides. 1993 Jan-Feb;14(1):1-5. doi: 10.1016/0196-9781(93)90003-y.
The immunosuppressor activity of the cycloamanides A, B, C, and D, and two of their D-amino acid residue-containing analogues, was examined using PFC (plaque forming cell) and DTH (delayed type hypersensitivity) tests. It was found that cycloamanide A (CyA A, II) [c-(Phe-Phe-Ala-Gly-Pro-Val-)] and its D-Phe-containing analogue III [c-(Phe-D-Phe-Ala-Gly-Pro-Val-)] are the most potent immunosuppressors of the whole series. The retroanalogue of III [c-(D-Phe-Val-Pro-Gly-Ala-)] was found to be less active than III. The immunosuppressor activity of O-carboxymethyl-Tyr6-antamanide (I) was also tested. It was found that the substitution of one of the Phe residues of ANT by O-carboxymethyl-Tyr does not substantially affect the immunosuppressor activity.
使用PFC（空斑形成细胞）和DTH（迟发型超敏反应）试验检测了环鹅膏肽A、B、C和D及其两种含D-氨基酸残基类似物的免疫抑制活性。发现环鹅膏肽A（CyA A，II）[c-(苯丙氨酸-苯丙氨酸-丙氨酸-甘氨酸-脯氨酸-缬氨酸-)]及其含D-苯丙氨酸的类似物III [c-(苯丙氨酸-D-苯丙氨酸-丙氨酸-甘氨酸-脯氨酸-缬氨酸-)]是整个系列中最有效的免疫抑制剂。发现III的反向类似物[c-(D-苯丙氨酸-缬氨酸-脯氨酸-甘氨酸-丙氨酸-)]的活性低于III。还测试了O-羧甲基-Tyr6-抗鹅膏肽（I）的免疫抑制活性。发现用O-羧甲基-Tyr取代ANT的一个苯丙氨酸残基不会实质性影响免疫抑制活性。
