Omar M S
Tropenmed Parasitol. 1978 Dec;29(4):462-72.
Histochemical differentiation of Onchocerca volvulus microfilariae from 164 patients in West African rain-forest (Liberia), Sudan-savanna (Upper Volta), Guatemala and the Yemen has been carried out using a staining method for the demonstration of acid phosphatase. Intrauterine microfilariae showed considerable changes in their enzyme activity during embryonic development which are probably associated with the maturation of the parasite before migration to the tissues. Five distinct types of staining patterns could be distinguished among microfilariae from the skin according to the localization of the enzyme in specific structures of the microfilaria. Two or more types of staining patterns were found in most persons in the different geographic regions. There were significant differences in the overall distribution of the various staining patterns in persons from the different areas. At the present state of our knowledge, little is known about the nature and significance of these differences in the staining patterns of microfilariae. The question of whether they can be ascribed to an ageing process, strain differences or other factors is discussed.
采用酸性磷酸酶染色法，对来自西非雨林地区（利比里亚）、苏丹稀树草原地区（上沃尔特）、危地马拉和也门的164例患者体内的盘尾丝虫微丝蚴进行了组织化学鉴别。子宫内微丝蚴在胚胎发育过程中其酶活性呈现出显著变化，这可能与寄生虫在迁移至组织之前的成熟过程有关。根据酶在微丝蚴特定结构中的定位，可在皮肤微丝蚴中区分出五种不同类型的染色模式。在不同地理区域的大多数人身上发现了两种或更多种染色模式。不同地区人群中各种染色模式的总体分布存在显著差异。就我们目前的知识水平而言，对于微丝蚴染色模式这些差异的性质和意义了解甚少。文中讨论了这些差异是否可归因于衰老过程、虫株差异或其他因素的问题。
