Okiji T, Morita I, Kawashima N, Kosaka T, Suda H, Murota S
Department of Endodontics, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Arch Oral Biol. 1993 Jan;38(1):31-6. doi: 10.1016/0003-9969(93)90151-b.
Localization of prostaglandin (PG) I2 synthase immunoreactivity was examined in demineralized sections of rat pulpal, periodontal and skeletal tissues using isn-1, a monoclonal antibody raised against the enzyme. Various calcified tissue-forming cells, i.e. odontoblasts, osteoblasts, osteocytes, cementoblasts, cementocytes and chondrocytes, were similarly immunoreactive for PGI2 synthase, suggesting that they are capable of producing PGI2. In odontoblasts and chondrocytes, the reactivity increased gradually with maturation. Weak immunoreactivity was also observed in endothelial cells and fibroblast-like cells in pulpal and periodontal tissues. However, no reactivity was seen in ameloblasts. These results suggest the possible involvement of PGI2 in the regulation of the metabolism of various calcified tissues. Monoclonal antibodies such as isn-1 may become useful markers of the maturation of calcified tissue-forming cells of mesenchymal origin.
使用针对该酶产生的单克隆抗体isn-1，在大鼠牙髓、牙周和骨骼组织的脱钙切片中检测了前列腺素（PG）I2合酶免疫反应性的定位。各种钙化组织形成细胞，即成牙本质细胞、成骨细胞、骨细胞、成牙骨质细胞、牙骨质细胞和软骨细胞，对PGI2合酶具有相似的免疫反应性，表明它们能够产生PGI2。在成牙本质细胞和软骨细胞中，反应性随着成熟而逐渐增加。在牙髓和牙周组织的内皮细胞和成纤维细胞样细胞中也观察到弱免疫反应性。然而，在成釉细胞中未观察到反应性。这些结果表明PGI2可能参与各种钙化组织代谢的调节。诸如isn-1之类的单克隆抗体可能成为间充质来源的钙化组织形成细胞成熟的有用标志物。
