冻干环氧乙烷处理的骨-髌腱-骨同种异体移植磨损颗粒诱导白细胞介素-1的产生：一项体外研究。

The induction of IL-1 by freeze-dried ethylene oxide-treated bone-patellar tendon-bone allograft wear particles: an in vitro study.

作者信息

Silvaggio V J, Fu F H, Georgescu H I, Evans C H

机构信息

Ferguson Laboratory for Orthopaedic Research, University of Pittsburgh School of Medicine, Pennsylvania 15261.

出版信息

Arthroscopy. 1993;9(1):82-6. doi: 10.1016/s0749-8063(05)80349-4.

DOI:10.1016/s0749-8063(05)80349-4

PMID:8442836

Abstract

There have been recent reports of adverse clinical results with freeze-dried ethylene oxide-treated bone-patellar tendon-bone (FD-ETO-BPTB) allografts used in anterior cruciate ligament (ACL) reconstruction. Ethylene oxide and its residues were implicated as the cause of many of the failures. Wear particles generated from both freeze-dried ethylene oxide-treated and deep frozen bone-patellar tendon-bone (DF-BPTB) allografts were placed in culture with lapine synoviocytes. The resulting synovial-conditioned media were then assayed for interleukin-1 (IL-1) content. IL-1 is a potent mediator of tissue inflammation. FD-ETO-BPTB wear particles generated statistically significant levels of IL-1 when compared with both a negative control and DF-BPTB wear particles.

摘要

最近有报道称，用于前交叉韧带（ACL）重建的冻干环氧乙烷处理的骨-髌腱-骨（FD-ETO-BPTB）同种异体移植物出现了不良临床结果。环氧乙烷及其残留物被认为是许多失败案例的原因。将冻干环氧乙烷处理的和深冻骨-髌腱-骨（DF-BPTB）同种异体移植物产生的磨损颗粒与兔滑膜细胞一起培养。然后测定所得滑膜条件培养基中的白细胞介素-1（IL-1）含量。IL-1是组织炎症的强效介质。与阴性对照和DF-BPTB磨损颗粒相比，FD-ETO-BPTB磨损颗粒产生的IL-1水平具有统计学意义。