Ezine S, Lucas B, Vicari A, Dautigny N, Vasseur F, Penit C
U345 INSERM, CHU Necker-Enfants Malades, Paris, France.
Int Immunol. 1993 Jan;5(1):89-96. doi: 10.1093/intimm/5.1.89.
MRL-lpr/lpr mice have hypertrophied lymph nodes comprising CD4-CD8- T cells. In addition, they contain CD4+CD8- T cells co-expressing the CD45RA marker. The correlation between these two subpopulations has been difficult to assess. We analyzed the expression of CD45RA (with the RA3-2C2 antibody) in various thymic and peripheral T cell subsets, using three-color immunofluorescence. We showed that in lpr mice (i) a transient CD4+CD8- thymic subset co-expresses CD45RA during the course of the disease, and (ii) thymic as well as peripheral CD4-CD8- and CD4+CD8- T cells brightly express CD45RA; furthermore (iii) in the lymph nodes, during lymphadenopathy, CD4+CD8-CD45RA+ T cells show a broad range of the CD4 fluorescence intensity, and (iv) the increase in MHC class II expression is restricted to CD45RA-T cells of the thymus and lymph nodes of lpr mice. Taken together, these data suggest that the CD4+CD8-CD45RA+ population might generate the CD4-CD8- tumor cells. In addition, using the bromodeoxyuridine labeling technique, we demonstrate that these cells are not the result of increased proliferation.
MRL-lpr/lpr小鼠有肥大的淋巴结，其中包含CD4-CD8-T细胞。此外，它们还含有共表达CD45RA标志物的CD4+CD8-T细胞。这两个亚群之间的相关性一直难以评估。我们使用三色免疫荧光分析了各种胸腺和外周T细胞亚群中CD45RA（用RA3-2C2抗体）的表达。我们发现，在lpr小鼠中：（i）在疾病过程中，一个短暂的CD4+CD8-胸腺亚群共表达CD45RA；（ii）胸腺以及外周的CD4-CD8-和CD4+CD8-T细胞强烈表达CD45RA；此外，（iii）在淋巴结肿大期间，淋巴结中的CD4+CD8-CD45RA+T细胞显示出广泛的CD4荧光强度范围；（iv）MHC II类表达的增加仅限于lpr小鼠胸腺和淋巴结中的CD45RA-T细胞。综上所述，这些数据表明CD4+CD8-CD45RA+群体可能产生CD4-CD8-肿瘤细胞。此外，使用溴脱氧尿苷标记技术，我们证明这些细胞不是增殖增加的结果。
