van der Leij F R, Abeln E C, Hesseling-Meinders A, Feenstra W J
Department of Genetics, University of Groningen, Haren, Netherlands.
Plant Mol Biol. 1993 Feb;21(3):567-71. doi: 10.1007/BF00028815.
We identified an open reading frame (ORF) which is located closely behind the gene encoding granule-bound starch synthase (GBSS) of potato (Solanum tuberosum L.). The ORF ends with a perfect 43 bp direct repeat, which carries the stop triplet precisely at the beginning of the second repeat. The deduced protein shows homology with all known isoforms of plant beta-1,3-glucanases and beta-1,3-1,4-glucanases. Although the DNA sequence is unique in potato and tomato (Lycopersicon esculentum L.), no expression of the gene was found in these species. Taken together with the unusual codon usage and length of the predicted protein, this sequence could be a pseudogene.
我们鉴定出一个开放阅读框（ORF），它位于马铃薯（Solanum tuberosum L.）颗粒结合淀粉合酶（GBSS）编码基因的紧邻下游。该开放阅读框以一个完美的43bp直接重复序列结尾，该重复序列恰好在第二个重复序列的起始处携带终止三联体。推导的蛋白质与植物β-1,3-葡聚糖酶和β-1,3-1,4-葡聚糖酶的所有已知同工型具有同源性。尽管该DNA序列在马铃薯和番茄（Lycopersicon esculentum L.）中是独特的，但在这些物种中未发现该基因的表达。结合不寻常的密码子使用情况和预测蛋白质的长度，该序列可能是一个假基因。
