Palacios R, Samaridis J
Basel Institute for Immunology, Switzerland.
Blood. 1993 Mar 1;81(5):1222-38.
We have established in culture several nontransformed bone marrow clones (called PR) that show phenotypic and genotypic characteristics that distinguish them from totipotent stem cells and lineage-restricted Pro-T lymphocytes, Pro-B lymphocytes, and myeloid cell progenitors. In vivo and/or in vitro the PR clones give rise to T lymphocytes, B lymphocytes, and some myeloid-lineage cells, but they appear not to be able to generate cells of the erythroid lineage, nor can they rescue mice from a lethal dose of irradiation. We conclude that the PR clones are precursor cells representing an intermediate stage of development between the totipotential stem cell and lineage-restricted progenitor cells. The results described here support a model of blood cell formation in which stem cell differentiation is a progressive process marked by the stepwise loss of self renewal and functional potential. In addition, they provide evidence that cytokines and specialized microenvironments can direct the fate of the developing multipotent progenitor cells.
我们在培养中建立了几个未转化的骨髓克隆（称为PR），它们表现出的表型和基因型特征使其有别于全能干细胞以及谱系受限的前T淋巴细胞、前B淋巴细胞和髓系细胞祖细胞。在体内和/或体外，PR克隆可产生T淋巴细胞、B淋巴细胞和一些髓系谱系细胞，但它们似乎无法产生红系谱系细胞，也无法使小鼠从致死剂量的辐射中获救。我们得出结论，PR克隆是前体细胞，代表全能干细胞和谱系受限祖细胞之间的一个中间发育阶段。此处描述的结果支持一种血细胞形成模型，其中干细胞分化是一个渐进过程，其标志是自我更新和功能潜能的逐步丧失。此外，它们提供了证据表明细胞因子和特殊微环境可指导发育中的多能祖细胞的命运。
