Matsukawa Y, Marui N, Sakai T, Satomi Y, Yoshida M, Matsumoto K, Nishino H, Aoike A
Department of Preventive Medicine, Kyoto Prefectural University of Medicine, Japan.
Cancer Res. 1993 Mar 15;53(6):1328-31.
Genistein, an isoflavone, is a specific inhibitor of tyrosine kinase and topoisomerase II. However, its effect on cell growth is unknown. Therefore, we examined the effects of genistein on cell growth and cell cycle progression and compared its effects with other flavonoids. Genistein inhibited in a dose-dependent manner the growth of HGC-27 cells derived from human gastric cancer. Flow-cytometric analysis showed that genistein almost completely arrested the cell cycle progression at G2-M. This effect was reversible when genistein was removed from the culture medium. In contrast, other flavonoids such as flavone, luteolin, and the structurally similar daidzein arrested the cell cycle at G1. Consistent with the flow-cytometric analysis, microscopic observation showed that genistein did not increase the mitotic index, which supposes that genistein may arrest the cell cycle at G2 or early M. These results suggest that the G2-M arrest by genistein is a unique effect among flavonoids.
染料木黄酮是一种异黄酮，是酪氨酸激酶和拓扑异构酶II的特异性抑制剂。然而，其对细胞生长的影响尚不清楚。因此，我们研究了染料木黄酮对细胞生长和细胞周期进程的影响，并将其与其他类黄酮的作用进行了比较。染料木黄酮以剂量依赖的方式抑制源自人胃癌的HGC-27细胞的生长。流式细胞术分析表明，染料木黄酮几乎完全使细胞周期进程停滞在G2-M期。当从培养基中去除染料木黄酮时，这种作用是可逆的。相比之下，其他类黄酮如黄酮、木犀草素以及结构相似的大豆苷元则使细胞周期停滞在G1期。与流式细胞术分析结果一致，显微镜观察显示染料木黄酮并未增加有丝分裂指数，这表明染料木黄酮可能使细胞周期停滞在G2期或M期早期。这些结果表明，染料木黄酮导致的G2-M期停滞是类黄酮中的一种独特作用。
