Aránega A E, Reina A, Muros M A, Alvarez L, Prados J, Aránega A
Department of Morphological Sciences, School of Medicine, University of Granada, Spain.
Int J Cardiol. 1993 Jan;38(1):49-55. doi: 10.1016/0167-5273(93)90203-s.
We used Western-blot analysis to investigate the possible presence in the bloodstream of the contractile protein alpha-actin in 70 patients diagnosed with acute myocardial infarction on the basis of clinical, electrocardiographic and laboratory (creatine kinase and lactate dehydrogenase) criteria. Circulating protein was identified with a monoclonal antibody specific for cardiac alpha-actin. Of the 70 control samples of blood, the immunoblot results were negative for alpha-actin in 98% of the cases. Of the 30 patients with skeletal muscle damage caused by surgery, 26 were negative for circulating alpha-actin. Of the 70 patients with acute myocardial infarction, circulating alpha-actin was found in 67 (95%) as a 43 kDa band in immunoblots; the highest circulating concentrations (0.0580 micrograms/microliters) were found in those with anterior acute myocardial infarction. Circulating alpha-actin was detected in samples taken between 1 and 180 h after the onset of pain, and showed a biphasic pattern of appearance. Our findings for serum alpha-actin, together with the relationship between serum concentrations of this protein and sex (p = 0.001), tobacco use (p = 0.007) and postepisode complications (p = 0.002), should make it possible to gain a deeper understanding of acute myocardial infarction as a clinical entity.
我们采用蛋白质印迹分析，以临床、心电图及实验室（肌酸激酶和乳酸脱氢酶）标准为依据，对70例诊断为急性心肌梗死的患者，研究其血液中收缩蛋白α-肌动蛋白的可能存在情况。用针对心脏α-肌动蛋白的单克隆抗体识别循环蛋白。在70份对照血样中，98%的免疫印迹结果显示α-肌动蛋白呈阴性。在30例因手术导致骨骼肌损伤的患者中，26例循环α-肌动蛋白呈阴性。在70例急性心肌梗死患者中，67例（95%）在免疫印迹中发现循环α-肌动蛋白呈43 kDa条带；在前壁急性心肌梗死患者中循环浓度最高（0.0580微克/微升）。在疼痛发作后1至180小时采集的样本中检测到循环α-肌动蛋白，其呈现双相出现模式。我们关于血清α-肌动蛋白的研究结果，以及该蛋白血清浓度与性别（p = 0.001）、吸烟情况（p = 0.007）和发作后并发症（p = 0.002）之间的关系，应有助于更深入地了解急性心肌梗死这一临床实体。
