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呼吸道合胞病毒mRNA 5'末端帽结构

The structure of the 5' terminal cap of the respiratory syncytial virus mRNA.

作者信息

Barik S

机构信息

Department of Molecular Biology, Cleveland Clinic Foundation, Ohio 44195.

出版信息

J Gen Virol. 1993 Mar;74 ( Pt 3):485-90. doi: 10.1099/0022-1317-74-3-485.

DOI:10.1099/0022-1317-74-3-485
PMID:8445369
Abstract

The 5' terminal cap structure of the mRNAs of human respiratory syncytial virus synthesized in vitro in the presence of S-adenosyl-L-methionine consists of a 7-methyl guanosine linked to an unmethylated guanosine through a 5'-5' pyrophosphate linkage formed by using the alpha and beta phosphates of GTP. The complete cap structure is m7G (5')ppp(5')Gp ... which is devoid of ribose 2'-O-methylation. Capping, including methylation, is coupled to transcription. These results constitute the first report of a pneumoviral mRNA cap structure.

摘要

在S-腺苷-L-甲硫氨酸存在的情况下体外合成的人呼吸道合胞病毒mRNA的5'末端帽结构由一个7-甲基鸟苷通过使用GTP的α和β磷酸形成的5'-5'焦磷酸键与一个未甲基化的鸟苷相连组成。完整的帽结构是m7G(5')ppp(5')Gp……其缺乏核糖2'-O-甲基化。包括甲基化在内的加帽与转录偶联。这些结果构成了肺炎病毒mRNA帽结构的首次报道。

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