Singh M, Meisner D, Faulkner G, Mezei M
College of Pharmacy, Dalhousie University, Halifax NS, Canada.
J Microencapsul. 1993 Jan-Mar;10(1):35-44. doi: 10.3109/02652049309015310.
Colloidal gold was used as an electron-dense marker for multilamellar vesicles to study the mechanism of liposome drug delivery to the eye and lung. A gold labelled multilamellar vesicle could be seen in the conjunctiva but there was no evidence of vesicles adsorbed to the epithelial surface of cornea or conjunctiva. In the lung, a free gold particle was isolated in type 1 epithelial cells and many vesicular structures were observed in the alveolar spaces which were not gold labelled. Experiments performed so far indicate that adsorption and not endocytosis was the major mechanism of uptake of drug or marker for multilamellar vesicles except for conjunctiva.
胶体金被用作多层囊泡的电子致密标记物，以研究脂质体药物向眼和肺递送的机制。在结膜中可以看到金标记的多层囊泡，但没有证据表明囊泡吸附在角膜或结膜的上皮表面。在肺中，在I型上皮细胞中分离出一个游离的金颗粒，并且在肺泡腔中观察到许多未被金标记的囊泡结构。迄今为止进行的实验表明，除结膜外，多层囊泡摄取药物或标记物的主要机制是吸附而非内吞作用。
