Crystallization and preliminary crystallographic data for formyltetrahydrofolate synthetase from Clostridium thermoaceticum.
作者信息
Lewinski K, Hui Y, Jakob C G, Lovell C R, Lebioda L
机构信息
Department of Chemistry and Biochemistry, University of South Carolina, Columbia 29208.
出版信息
J Mol Biol. 1993 Feb 20;229(4):1153-6. doi: 10.1006/jmbi.1993.1111.
PMID:8445641
Abstract
Formyltetrahydrofolate synthetase from Clostridium thermoaceticum has been crystallized using the hanging-drop method from ammonium sulphate and PEG solutions. Crystals are trigonal, the space group is R32, a = 163 A, c = 259 A. A 4.2 A resolution data set has been collected. Analysis of the data using the self-rotation function shows that tetramers have approximate 222 symmetry and are positioned on a crystallographic 2-fold axis.
摘要
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