（D-丙氨酸，D-亮氨酸）脑啡肽可降低海马体膜中鸟苷三磷酸（GTP）的结合。

(D-Ala, D-Leu) enkephalin reduces the binding of GTP in hippocampal membranes.

作者信息

Aramaki T, Saito K, Shinno E, Ohnishi T, Ooi Y, Maeda S, Inoki R

机构信息

Department of Pharmacology, Faculty of Dentistry, Osaka University, Suita, Japan.

出版信息

Life Sci. 1993;52(11):901-6. doi: 10.1016/0024-3205(93)90524-7.

DOI:10.1016/0024-3205(93)90524-7

PMID:8445984

Abstract

The effect of (D-Ala, D-Leu) enkephalin (DADLE) on the binding of GTP in hippocampal preparations was studied. It was observed that treatment of hippocampal slices with 10(-5) -5 x 10(-5) M DADLE followed by the preparation of membrane fractions reduced the binding of 35S-GTP-gamma-S. There was no change in the affinity of the binding. This decrease of 35S-GTP-gamma-S binding was reversed when 5 x 10(-5) M naltrindole was included. The effect was not observed when the membrane fractions were incubated with DADLE. Photoaffinity labeling with the use of 32P P3-(4-azidoanilido)-P1 5'-GTP followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography revealed the incorporation of radioactivity into molecular mass of the 43 kDa and 33-34 kDa proteins. 32P Photolabeling of both the 43 kDa and 33-34 kDa bands decreased following treatment of hippocampal slices with 10(-4) M DADLE. These results suggested that DADLE reduces the GDP-GTP exchange in hippocampal membranes.

摘要

研究了（D-丙氨酸，D-亮氨酸）脑啡肽（DADLE）对海马制剂中GTP结合的影响。观察到用10^(-5)-5×10^(-5) M DADLE处理海马切片，随后制备膜组分，会降低35S-GTP-γ-S的结合。结合亲和力没有变化。当加入5×10^(-5) M纳曲吲哚时，35S-GTP-γ-S结合的这种降低被逆转。当膜组分与DADLE一起孵育时未观察到该效应。使用32P P3-(4-叠氮苯胺基)-P1 5'-GTP进行光亲和标记，随后进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和放射自显影，结果显示放射性掺入到43 kDa和33-34 kDa蛋白质的分子量中。用10^(-4) M DADLE处理海马切片后，43 kDa和33-34 kDa条带的32P光标记均减少。这些结果表明，DADLE减少了海马膜中的GDP-GTP交换。