Montgomerie J Z, Holshuh H J, Keyser A J, Bennett C J, Schick D G
Department of Infectious Diseases, Rancho Los Amigos Medical Center, Downey, California 90242.
Paraplegia. 1993 Feb;31(2):105-10. doi: 10.1038/sc.1993.18.
Antibody to 28 K was used to examine sections of bladder biopsies obtained by cystoscopy from 14 patients with spinal cord injury (SCI). Most of the biopsies were obtained from patients with indwelling catheters during the investigation for possible malignancy. Sections of bladder were stained by the streptavidin procedure. The 28 K in the normal transitional epithelium of the bladder was in the superficial cells (umbrella cells). All the biopsies from patients with indwelling urethral catheters showed areas of squamous metaplasia usually associated with evidence of chronic inflammation. Cystitis cystica glandularis was also seen in one patient. Staining was most marked in the areas of squamous metaplasia with intracellular granular staining. The basal layers were not well stained. With marked squamous metaplasia, there was a superficial hyperkeratotic layer that stained variably and often did not stain at all. Staining was less marked in areas of hyperplasia, regenerating urothelium, and cystitis cystica glandularis. These findings raise the possibility that the presence of 28 K glycoprotein in the tissues or released into the urine may be used as an indicator of squamous metaplasia and chronic inflammation of the bladder.
用抗28K抗体检测了14例脊髓损伤（SCI）患者经膀胱镜检查获取的膀胱活检组织切片。大多数活检组织是在对可能存在的恶性肿瘤进行调查期间从留置导尿管的患者身上获取的。膀胱切片采用链霉亲和素法染色。膀胱正常移行上皮中的28K存在于表层细胞（伞细胞）中。所有来自留置尿道导管患者的活检组织均显示有鳞状化生区域，通常伴有慢性炎症证据。在1例患者中还发现了腺性膀胱炎。染色在鳞状化生区域最为明显，呈细胞内颗粒状染色。基底层染色不佳。在明显的鳞状化生区域，有一层浅表角化过度层，染色不一，且常常根本不着色。在增生、再生的尿路上皮和腺性膀胱炎区域，染色不那么明显。这些发现提示，组织中或释放到尿液中的28K糖蛋白的存在可能用作膀胱鳞状化生和慢性炎症的指标。
