Jenkins M Y, Sheikh N M, Mitchell G V, Grundel E, Blakely S R, Carter C J
Division of Nutrition, Food Safety and Applied Nutrition, U.S. Food and Drug Administration, Washington, DC 20204.
Nutr Cancer. 1993;19(1):55-65. doi: 10.1080/01635589309514236.
In a study of the effects of carotenoids, canthaxanthin (CA), beta-apo-8'-carotenal (BA), or beta-carotene in an extract of Spirulina-Dunaliella algae (AE) was fed at 0%, 0.1%, or 0.2% in a choline-deficient (CD) diet. In each of eight groups, 10 adult male Fischer 344 rats were fed diets with designated carotenoid sources and levels or a choline-sufficient diet for 12 weeks. Carotenoids altered some of the changes induced by the CD diet. Increases in enlargement of fatty livers and low plasma cholesterol levels occurred in rats fed 0.2% BA. Plasma retinol was further reduced 35% by BA or AE. BA and AE increased liver total vitamin A about 80% and 305%, respectively. Liver lipid peroxidation was enhanced and plasma alpha-tocopherol was reduced further by 1.0% AE. AE, BA, and CA (mg/g fat) depressed liver alpha-tocopherol about 49%, 67%, and 78%, respectively. The decreased liver alpha-tocopherol was concurrent with an increase in carotenoid stores of CA > BA > AE. Histopathological examination of sections of liver tissue by light microscopy showed fatty and cirrhotic changes in all rats fed CD diets. Histochemical evaluation based on a semiquantitative assay revealed a marked increase in peroxisome enzyme activity in the livers of all CD rats. None of the carotenoids appeared to have any effect on the development of morphological changes in the liver. Although carotenoids can function as antioxidants, they did not prevent changes observed in rats fed CD diets.
在一项关于类胡萝卜素作用的研究中，将螺旋藻 - 杜氏藻提取物（AE）中的角黄素（CA）、β - 阿朴 - 8'- 胡萝卜醛（BA）或β - 胡萝卜素以0%、0.1%或0.2%的比例添加到胆碱缺乏（CD）饮食中。在八组实验中，每组10只成年雄性Fischer 344大鼠分别喂食含有指定类胡萝卜素来源和水平的饮食或胆碱充足的饮食，持续12周。类胡萝卜素改变了CD饮食诱导的一些变化。喂食0.2% BA的大鼠出现脂肪肝肿大增加和血浆胆固醇水平降低。BA或AE使血浆视黄醇进一步降低了35%。BA和AE分别使肝脏总维生素A增加了约80%和305%。1.0% AE进一步增强了肝脏脂质过氧化作用，并使血浆α - 生育酚降低。AE、BA和CA（毫克/克脂肪）分别使肝脏α - 生育酚降低了约49%、67%和78%。肝脏α - 生育酚的降低与CA > BA > AE的类胡萝卜素储存增加同时发生。通过光学显微镜对肝组织切片进行组织病理学检查发现，所有喂食CD饮食的大鼠都出现了脂肪性和肝硬化变化。基于半定量分析的组织化学评估显示，所有CD大鼠肝脏中的过氧化物酶活性显著增加。没有一种类胡萝卜素似乎对肝脏形态变化的发展有任何影响。尽管类胡萝卜素可以作为抗氧化剂发挥作用，但它们并不能预防喂食CD饮食的大鼠中观察到的变化。
