Collin M, Herbein G, Montaner L, Gordon S
University of Oxford, Sir William Dunn School of Pathology, UK.
Res Virol. 1993 Jan-Feb;144(1):13-9. doi: 10.1016/s0923-2516(06)80006-3.
Amplification of early reverse transcripts by the polymerase chain reaction has been used to measure HIV1 entry into H9 cells and primary macrophages. With a single-step method of DNA preparation, we performed time course experiments to follow the appearance of long terminal repeat DNA. In both cell types, the formation of DNA was completely inhibited by anti-CD4 antibody, confirming the requirement of CD4 for virus binding and entry, and demonstrating that there were no CD4-independent routes of infection. In agreement with previous infectivity studies, sCD4 inhibited the entry of IIIB into H9, but only partially blocked Ba-L into macrophage when used at a comparable dose.
通过聚合酶链反应扩增早期逆转录产物已被用于检测HIV-1进入H9细胞和原代巨噬细胞的情况。采用单步DNA制备方法，我们进行了时间进程实验以追踪长末端重复序列DNA的出现。在这两种细胞类型中，DNA的形成均被抗CD4抗体完全抑制，这证实了病毒结合和进入需要CD4，并表明不存在不依赖CD4的感染途径。与先前的感染性研究一致，可溶性CD4（sCD4）抑制IIIB毒株进入H9细胞，但以相当剂量使用时，仅部分阻断Ba-L毒株进入巨噬细胞。
