Hardarson S, Labrecque D R, Mitros F A, Neil G A, Goeken J A
Department of Pathology, University of Iowa, Iowa City.
Am J Clin Pathol. 1993 Mar;99(3):277-81. doi: 10.1093/ajcp/99.3.277.
The prevalence of antineutrophil cytoplasmic antibodies was evaluated in patients with ulcerative colitis, primary sclerosing cholangitis, and various other gastrointestinal and hepatobiliary diseases to define the sensitivity and specificity of the test. The presence of antineutrophil cytoplasmic antibodies was detected in alcohol-fixed cytospin preparations of peripheral blood neutrophils with an indirect immunofluorescence technique. A perinuclear staining pattern was considered positive. Thirty-six of 50 patients (72%) with ulcerative colitis and/or primary sclerosing cholangitis had positive results. Twenty-two of 210 patients (10%) in the control group had positive findings, including a significant proportion of patients with autoimmune hepatitis (50%) and non-A, non-B and non-C hepatitis (27%). This test for antineutrophil cytoplasmic antibodies has a sensitivity of 72% and specificity of 90% for either ulcerative colitis or primary sclerosing cholangitis. It may be useful in the differential diagnosis of Crohn's disease and ulcerative colitis and in the early diagnosis of ulcerative colitis. It also may be employed to distinguish primary biliary cirrhosis from primary sclerosing cholangitis.
为明确抗中性粒细胞胞浆抗体检测的敏感性和特异性，对溃疡性结肠炎、原发性硬化性胆管炎以及其他各种胃肠道和肝胆疾病患者进行了抗中性粒细胞胞浆抗体患病率评估。采用间接免疫荧光技术，在外周血中性粒细胞的酒精固定甩片标本中检测抗中性粒细胞胞浆抗体的存在。核周染色模式被视为阳性。50例溃疡性结肠炎和/或原发性硬化性胆管炎患者中有36例（72%）结果呈阳性。210例对照组患者中有22例（10%）结果呈阳性，其中包括相当比例的自身免疫性肝炎患者（50%）以及非甲、非乙、非丙型肝炎患者（27%）。抗中性粒细胞胞浆抗体检测对溃疡性结肠炎或原发性硬化性胆管炎的敏感性为72%，特异性为90%。该检测在克罗恩病和溃疡性结肠炎的鉴别诊断以及溃疡性结肠炎的早期诊断中可能有用。它还可用于区分原发性胆汁性肝硬化和原发性硬化性胆管炎。
