Pilegaard H, Juel C, Wibrand F
August Krogh Institute, University of Copenhagen, Denmark.
Am J Physiol. 1993 Feb;264(2 Pt 1):E156-60. doi: 10.1152/ajpendo.1993.264.2.E156.
The effect of training on lactate transport capacity was studied in giant vesicles obtained with collagenase treatment of rat skeletal muscles. Marker enzyme analyses demonstrated that these vesicles are predominantly of sarcolemmal origin. Treadmill training induced significant adaptations in the capacity of rat skeletal muscles to transport lactate but swimming [low-intensity training, approximately 50% of maximal oxygen consumption (VO2 max)] did not. After 7 wk of moderate (90% of VO2max)- and high-intensity (112% of VO2max) interval treadmill training the carrier-mediated equilibrium exchange flux with 30 mM lactate was increased by 58 and 76%, respectively. During 5 wk of detraining the capacity to transport lactate decreased to near control level. It is concluded that physical training can increase the capacity to transport lactate in rat skeletal muscles and that the training intensity is of great importance. The adaptation appears to include both an increased number of transport proteins and a higher affinity of the individual transporters.
利用胶原酶处理大鼠骨骼肌获得的巨型囊泡，研究了训练对乳酸转运能力的影响。标记酶分析表明，这些囊泡主要来源于肌膜。跑步机训练可使大鼠骨骼肌转运乳酸的能力产生显著适应性变化，但游泳（低强度训练，约为最大耗氧量VO₂ max］的 50%）则不会。经过 7 周的中等强度（VO₂ max 的 90%）和高强度（VO₂ max 的 112%）间歇跑步机训练后，与 30 mM 乳酸的载体介导平衡交换通量分别增加了 58%和 76%。在 5 周的停训期间，乳酸转运能力降至接近对照水平。得出的结论是，体育锻炼可提高大鼠骨骼肌转运乳酸的能力，且训练强度至关重要。这种适应性变化似乎包括转运蛋白数量的增加和单个转运体更高的亲和力。
