巴氏醋杆菌质粒pAC1复制子的特性分析。
Characterization of the replicon from plasmid pAC1 from Acetobacter pasteurianus.
作者信息
Grones J, Králová A, Turna J
机构信息
Department of Molecular Biology, Faculty of Natural Science, Comenius University, Bratislava, Czech and Slovak Federal Republic.
出版信息
Biochem Biophys Res Commun. 1993 Feb 26;191(1):26-31. doi: 10.1006/bbrc.1993.1179.
A panel of recombinant plasmids pACK5 and pACT7 was prepared by introducing kanamycin and tetracycline resistance into the partially split plasmid pAC1 which contained replicon isolated from Acetobacter pasteurianus. The replicon in plasmid pAC1 is compatible with the ColE1 replicon. Compared to pBR322, the plasmid had more than 30 copies per chromosome in Escherichia coli cells. Plasmids were transformed into E. coli DH1, Acetobacter pasteurianus 3614, Acetobacter aceti 3620, Shigella, Citrobacter, and Brevibacterium flavum cells, and the stability of plasmid DNA was tested after cultivation in nonselective conditions.
通过将卡那霉素和四环素抗性引入部分裂解的质粒pAC1中制备了一组重组质粒pACK5和pACT7，pAC1含有从巴氏醋杆菌分离的复制子。质粒pAC1中的复制子与ColE1复制子兼容。与pBR322相比，该质粒在大肠杆菌细胞中每条染色体有30多个拷贝。将质粒转化到大肠杆菌DH1、巴氏醋杆菌3614、醋酸醋杆菌3620、志贺氏菌、柠檬酸杆菌和黄色短杆菌细胞中，并在非选择性条件下培养后测试质粒DNA的稳定性。
Zotero 插件