成年大鼠心室心肌细胞原强啡肽mRNA表达情况

Prodynorphin mRNA expression in adult cultured rat ventricular cardiac myocytes.

作者信息

Canossa M, Ventura C, Vaona I, Carboni L, Guarnieri C, Spampinato S

机构信息

Department of Pharmacology, University of Bologna, Italy.

出版信息

Biochim Biophys Acta. 1993 Mar 20;1172(3):247-50. doi: 10.1016/0167-4781(93)90210-5.

DOI:10.1016/0167-4781(93)90210-5

PMID:8448203

Abstract

Prodynorphin mRNA was synthesized both in rat atrial and ventricular tissue, as well as in adult cultured rat ventricular cardiac myocytes. In the cultured cells, the content of prodynorphin mRNA did not differ from that detected in the original ventricle, indicating that the myocardial cell is an important source for prodynorphin mRNA in the rat ventricular tissue. This study demonstrated the presence of immunoreactive dynorphin B-like material in the cultured cardiomyocytes. Gel permeation chromatography analysis of this material revealed the presence of forms with an apparently higher molecular weight than authentic dynorphin B.

摘要

前强啡肽原mRNA在大鼠心房和心室组织以及成年大鼠心室心肌细胞的培养物中均有合成。在培养细胞中，前强啡肽原mRNA的含量与原始心室中检测到的含量没有差异，这表明心肌细胞是大鼠心室组织中前强啡肽原mRNA的重要来源。本研究证明了在培养的心肌细胞中存在免疫反应性强啡肽B样物质。对该物质的凝胶渗透色谱分析显示，存在分子量明显高于天然强啡肽B的形式。