Schmidt M, Pichl L, Lepper M, Feierabend J
Botanisches Institut, J.W. Goethe-Universität, Frankfurt am Main, Germany.
Biochim Biophys Acta. 1993 Mar 20;1172(3):349-52. doi: 10.1016/0167-4781(93)90230-b.
Two different cDNA clones (SCL12-1 and SCL12-2) encoding precursors of a chloroplast ribosomal protein with homology to L12 from Escherichia coli were isolated from rye leaf cDNA libraries and sequenced. The corresponding polypeptide of rye chloroplast ribosomes was identified. The sequences for the mature proteins of M(r) 13,447 and 13,609 share 85% amino acid identity. The mature polypeptide of clone SCL12-1 has an amino acid identity of 71%, 72% or 44%, respectively, relative to L12 proteins from spinach, tobacco, or E. coli. Codon usage of the rye L12 cDNAs shows a high preference (97% and 82%) for G or C in the third base position.
从黑麦叶片cDNA文库中分离出两个不同的cDNA克隆（SCL12 - 1和SCL12 - 2），它们编码一种与大肠杆菌L12具有同源性的叶绿体核糖体蛋白前体，并进行了测序。鉴定出了黑麦叶绿体核糖体的相应多肽。分子量为13447和13609的成熟蛋白序列具有85%的氨基酸同一性。相对于菠菜、烟草或大肠杆菌的L12蛋白，克隆SCL12 - 1的成熟多肽分别具有71%、72%或44%的氨基酸同一性。黑麦L12 cDNA的密码子使用情况显示，在第三位碱基位置对G或C有很高的偏好性（分别为97%和82%）。
