Liu E T, He M, Rajgopal U
Department of Medicine, University of North Carolina, Chapel Hill 27599.
Semin Cancer Biol. 1993 Feb;4(1):47-58.
Abnormalities of gene dosage are important in human disease. We have developed the differential polymerase chain reaction (PCR) to detect gene amplification and deletion in small amounts of tissues whose DNA may be degraded. The current utility of differential PCR required optimization of DNA isolation procedures, of primer selection approaches, and of PCR conditions. From this experience, we have formulated a theoretical framework for the technique, and describe appropriate applications and potential improvements based on emerging PCR technologies. Special emphasis is placed on the analysis of formalin fixed and paraffin embedded, archived specimens.
基因剂量异常在人类疾病中很重要。我们开发了差异聚合酶链反应（PCR）来检测少量DNA可能已降解的组织中的基因扩增和缺失。差异PCR的当前应用需要优化DNA分离程序、引物选择方法和PCR条件。基于这一经验，我们为该技术构建了一个理论框架，并描述了基于新兴PCR技术的适当应用和潜在改进。特别强调对福尔马林固定石蜡包埋的存档标本的分析。
