离子色谱法测定血浆草酸盐的再探讨。

Ion-chromatographic determination of plasma oxalate reexamined.

作者信息

Petrarulo M, Cerelli E, Marangella M, Maglienti F, Linari F

机构信息

Renal Stone Laboratory, Mauriziano Hospital Umberto I, Turin, Italy.

出版信息

Clin Chem. 1993 Mar;39(3):537-9.

PMID:8448873

Abstract

This new procedure for determining oxalic acid in plasma is based on sample deproteinization with hydrochloric acid and acetonitrile and subsequent ion-chromatographic assay of the neutralized supernate. Sample pretreatment produces very clean samples, which ensures long column life. Mean analytical recovery of oxalate (5.0-10.0 mumol/L) added to plasma samples averaged 98.6 +/- 6.2%; imprecision (CV) was 5.2% (at 2.2 mumol/L) and the detection limit was 0.5 mumol/L at a signal-to-noise ratio of 5:1. Ascorbate to oxalate conversion was < 0.2%, indicating that the procedure is free from ascorbate interference. Plasma oxalate concentrations, measured in samples from 31 healthy persons, ranged from 0.8 to 3.4 mumol/L (mean 1.89, SD 0.75 mumol/L), which agrees with results from indirect radioisotopic dilution methods.

摘要

这种测定血浆中草酸的新方法基于用盐酸和乙腈对样品进行脱蛋白处理，然后对中和后的上清液进行离子色谱分析。样品预处理产生非常纯净的样品，这确保了色谱柱的长使用寿命。添加到血浆样品中的草酸盐（5.0 - 10.0 μmol/L）的平均分析回收率为98.6±6.2%；不精密度（CV）为5.2%（在2.2 μmol/L时），在信噪比为5:1时检测限为0.5 μmol/L。抗坏血酸向草酸盐的转化率<0.2%，表明该方法不受抗坏血酸干扰。在31名健康人的样品中测得的血浆草酸盐浓度范围为0.8至3.4 μmol/L（平均值1.89，标准差0.75 μmol/L），这与间接放射性同位素稀释法的结果一致。