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急性剂量的中波紫外线照射后虹鳟(Oncorhyncus mykiss)晶状体受到的损伤。

Damage to the rainbow trout (Oncorhyncus mykiss) lens following an acute dose of UVB.

作者信息

Cullen A P, Monteith-McMaster C A

机构信息

Optical Radiation Laboratory, School of Optometry, University of Waterloo, Ontario, Canada.

出版信息

Curr Eye Res. 1993 Feb;12(2):97-106. doi: 10.3109/02713689308999477.

DOI:10.3109/02713689308999477
PMID:8449030
Abstract

Rainbow trout (Oncorhynchus mykiss) were used to model the solar radiation-induced cataract that occurs in hatchery-reared lake trout (Salvelinus namaycush). Rainbow trout eyes were exposed to wideband predominantly UVB radiant energy from a filtered 1000W xenon source. Lenticular damage was assessed and classified and comeal damage noted. Lenticular threshold radiant exposure was 0.48 J.cm-2 which is in the same range as the mammalian lens. Permanent lenticular damage occurred at radiant exposure levels approximately twice the threshold for lenticular radiant exposure (also similar to the mammalian response). Slit-lamp and scanning electron microscopical results support the theory of post-exposure anterior subcapsular epithelial cell damage, lens fibre swelling, and eventual rupture, leading to areas of lower refractive index in the anterior subcapsular region of the lens.

摘要

虹鳟(Oncorhynchus mykiss)被用于模拟孵化场养殖的湖鳟(Salvelinus namaycush)中发生的太阳辐射诱导性白内障。虹鳟的眼睛暴露于来自经过滤的1000瓦氙光源的主要为UVB的宽带辐射能量。评估并分类晶状体损伤,并记录角膜损伤情况。晶状体阈值辐射暴露为0.48 J.cm-2,这与哺乳动物晶状体处于相同范围。在辐射暴露水平约为晶状体辐射暴露阈值两倍时发生永久性晶状体损伤(这也与哺乳动物的反应相似)。裂隙灯和扫描电子显微镜结果支持暴露后前囊下上皮细胞损伤、晶状体纤维肿胀并最终破裂的理论,这会导致晶状体前囊下区域出现较低折射率区域。

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