Marshall M O, Thomas H M, Seatter M J, Greer K R, Wood P J, Gould G W
Novo Nordisk ASA/S, Bagsvaerd, Denmark.
Biochem Soc Trans. 1993 Feb;21(1):164-8. doi: 10.1042/bst0210164.
The application of molecular biology to the study of membrane transport proteins has led to a rapid advance in our understanding of the mechanisms behind the regulation of blood glucose levels. Moreover the demonstration of lesions in the expression of GLUT2 in the islets from diabetic models has provided a focus for research efforts aimed at addressing the defects responsible for the development and onset of both type I and perhaps type II diabetes. The recent demonstration that an 'artificial beta-cell' can be engineered from anterior pituitary-derived cell lines by transfection with both the insulin cDNA and the cDNA encoding GLUT2 represents a significant advance in the development of potential therapies for type I diabetes [24].
分子生物学在膜转运蛋白研究中的应用，使我们对血糖水平调节机制的理解有了迅速进展。此外，在糖尿病模型胰岛中GLUT2表达损伤的证明，为旨在解决I型糖尿病以及可能的II型糖尿病发生和发病所涉及缺陷的研究工作提供了一个重点。最近有证据表明，通过用胰岛素cDNA和编码GLUT2的cDNA转染，可从前垂体来源的细胞系构建“人工β细胞”，这在I型糖尿病潜在治疗方法的开发方面是一项重大进展[24]。
