Finamore F J, Feldman R P, Serrano L J, Cosgrove G E
Int J Vitam Nutr Res. 1977;47(1):62-7.
Rabbits were randomly assigned to two groups. Age and body weight distribution were equal in both groups. All animals were placed on a high cholesterol diet for 9 wk, then returned to a normal diet for 1 wk. At the end of this dietary regimen, one group of animals received subcutaneous injections of psysiological saline 3 times/day, 5 days/wk for 10 wk, and the other group recieved L-ascorbate 2-sulfate (0.37 mmole) according to the same timetable. On alternate weeks, the serum levels of total and free cholesterol were determined. After 10 wk of treatment the animals were killed; the plaques were excised from the aortas and examined for total mass and cholesterol content. We observed that, under these dietary conditions, L-ascorbate 2-sulfate does not mobilize cholesterol or its esters from preformed aortic plaque. However, we did observe that animals showing high cholesterol levels in their sera died prematurely when injected with L-ascorbate 2-sulfate. Gross and histopathological investigations of organs and tissues did not reveal any significant differences among the animals that died prematurely and those that survived to the termination of the experiment.
兔子被随机分为两组。两组的年龄和体重分布相同。所有动物先接受9周的高胆固醇饮食，然后恢复1周的正常饮食。在这种饮食方案结束时，一组动物每周5天、每天3次皮下注射生理盐水，共10周，另一组按照相同时间表接受L-抗坏血酸2-硫酸盐（0.37毫摩尔）。每隔一周测定血清总胆固醇和游离胆固醇水平。治疗10周后处死动物；从主动脉中切除斑块，检查其总质量和胆固醇含量。我们观察到，在这些饮食条件下，L-抗坏血酸2-硫酸盐不会从预先形成的主动脉斑块中动员胆固醇或其酯类。然而，我们确实观察到，血清胆固醇水平高的动物在注射L-抗坏血酸2-硫酸盐后过早死亡。对器官和组织的大体和组织病理学检查未发现过早死亡的动物与存活至实验结束的动物之间有任何显著差异。
