11号染色体11q11-q13区域十二个基因座的脉冲场凝胶电泳（PFGE）图谱。

A pulsed-field gel electrophoresis (PFGE) map of twelve loci on chromosome 11q11-q13.

作者信息

Petty E M, Arnold A, Marx S J, Bale A E

机构信息

Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.

出版信息

Genomics. 1993 Feb;15(2):423-5. doi: 10.1006/geno.1993.1080.

DOI:10.1006/geno.1993.1080

PMID:8449512

Abstract

We report a pulsed-field gel electrophoresis map of 12 loci on proximal human chromosome 11q. Linkage studies have shown that this region of chromosome 11 contains the genes for familial atopic disease (APY) and multiple endocrine neoplasia type I (MEN1) (4). A physical map containing polymorphic loci will aid in the isolation of these disease genes. The map reported here has two noncontiguous groups of loci accounting for 8 of the 12 loci evaluated. One group spans a maximum distance of 1600 kb and includes D11S146, BCL1, PRAD1, INT2, and HSTF1. The other group includes FTH1, C1NH, and COX8. TCN1, PGA, and PYGM did not yield any comigrating fragments and could not be physically linked on this PFGE map. These data enhance previously published physical maps of proximal 11q by refining the localization of and distances between markers in the BCL1 region. Additionally, new information about the locations and physical relationships between FTH1, C1NH, and COX8 is presented.

摘要

我们报告了人类近端11号染色体上12个位点的脉冲场凝胶电泳图谱。连锁研究表明，11号染色体的这个区域包含家族性特应性疾病（APY）和多发性内分泌肿瘤I型（MEN1）的基因（4）。包含多态性位点的物理图谱将有助于分离这些疾病基因。此处报告的图谱有两个不连续的位点组，占所评估的12个位点中的8个。一组跨度最大为1600 kb，包括D11S146、BCL1、PRAD1、INT2和HSTF1。另一组包括FTH1、C1NH和COX8。TCN1、PGA和PYGM未产生任何共迁移片段，在该脉冲场凝胶电泳图谱上无法进行物理连接。这些数据通过完善BCL1区域中标记的定位和距离，增强了先前发表的近端11q物理图谱。此外，还提供了关于FTH1、C1NH和COX8的位置及物理关系的新信息。