Nunziata B, Smith R S, Weimar V
Invest Ophthalmol Vis Sci. 1977 Apr;16(4):285-91.
Vascularization of the rat cornea was produced by means of radiofrequency burns adjacent to the limbus. Animals with and without lesions were tested for a vascular response to topical PGE1 and compound 48/80, with carbon used as an indicator. There was no response to PGE1 until 6 hours, at which time dense labeling of limbal vessels was observed. This response gradually decreased and by day 15, when labeling returned to control levels. No further permeability changes were seen for the duration of the experiment to day 90. Compound 48/80 gave four-plus labeling of the limbus and one-plus labeling of corneal vessels at all times during the experiment. The presence or absence of the thermal lesion did not change the degree of labeling. Histamine and bradykinin did not produce vascular labeling either with or without a corneal lesion.
通过在角膜缘附近进行射频烧灼来诱导大鼠角膜血管化。对有损伤和无损伤的动物进行测试，以观察其对局部应用前列腺素E1（PGE1）和化合物48/80的血管反应，使用碳作为指示剂。在6小时之前对PGE1无反应，此时观察到角膜缘血管有密集标记。这种反应逐渐减弱，到第15天时，标记恢复到对照水平。在实验持续到第90天期间，未观察到进一步的通透性变化。在实验期间的所有时间，化合物48/80均使角膜缘出现4+以上标记，角膜血管出现1+以上标记。热损伤的有无并未改变标记程度。组胺和缓激肽无论有无角膜损伤均未产生血管标记。
