Rompen E H, Kohl J, Nusgens B, Lapiere C M
Department of Dentistry, University of Liège, Belgium.
J Dent Res. 1993 Mar;72(3):607-12. doi: 10.1177/00220345930720030901.
An in vitro model has been developed for measurement of initial attachment of 3H-labeled human gingival fibroblasts (HGF) and periodontal ligament cells (HPDL cells) to sieved dentin powder. Reproducible attachment was obtained and was closely related to the number of plated cells, the amount of dentin powder, the temperature, and the time (from 0 to 6 h) of incubation. HGF and HPDL cells had a high affinity for untreated dentin. Heat-denatured BSA coating of the dentin fully inhibited cell attachment, while a fibronectin coating had no significant effect. A surface conditioning of the dentin grains by 3% citric acid or by 2.5% minocycline-HCl increased the initial attachment of HPDL cells significantly (p < 0.05). Minocycline-HCl was more efficient than citric acid in that respect. In addition, HGF attachment to untreated dentin was highly significantly improved (p < 0.005) by a pre-incubation of the cells with 50 micrograms/mL of minocycline, and there was also indication (p = 0.067) of improvement by the presence of minocycline in the attachment medium, with a maximum of efficacy at 110 micrograms/mL. These results suggest that minocycline-HCl directly influenced the attachment properties of fibroblastic cells and that citric acid and minocycline-HCl could act by different mechanisms. HGF and HPDL cells originating from the same patient displayed no significant difference in their attachment to dentin in this model.
已建立一种体外模型，用于测量³H标记的人牙龈成纤维细胞（HGF）和牙周膜细胞（HPDL细胞）对筛分牙本质粉末的初始附着情况。获得了可重复的附着结果，且该结果与接种细胞数量、牙本质粉末量、温度以及孵育时间（0至6小时）密切相关。HGF和HPDL细胞对未处理的牙本质具有高亲和力。牙本质的热变性牛血清白蛋白包被完全抑制细胞附着，而纤连蛋白包被则无显著影响。用3%柠檬酸或2.5%盐酸米诺环素对牙本质颗粒进行表面处理可显著增加HPDL细胞的初始附着（p<0.05）。在这方面，盐酸米诺环素比柠檬酸更有效。此外，用50微克/毫升的盐酸米诺环素预孵育细胞可显著提高HGF对未处理牙本质的附着（p<0.005），并且在附着培养基中存在盐酸米诺环素也有改善的迹象（p=0.067），在110微克/毫升时效果最佳。这些结果表明盐酸米诺环素直接影响成纤维细胞的附着特性，并且柠檬酸和盐酸米诺环素可能通过不同机制起作用。在该模型中，来自同一患者的HGF和HPDL细胞在对牙本质的附着方面无显著差异。
