Essenberg R C, Sharma Y K
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078.
J Gen Microbiol. 1993 Jan;139(1):87-93. doi: 10.1099/00221287-139-1-87.
By selecting for growth of Escherichia coli mutant strains in the absence of the required amino acid, clones were found in a Brucella abortus library carrying genes for glutamyl phosphate reductase (proA) and beta-isopropylmalate dehydrogenase (leuB). These clones hybridized to unique fragments in a genomic digest of B. abortus DNA. The proA-complementing DNA was found in a region of 1.3 kb, which directed the synthesis of a protein of 48,000 Da with a pI of 6.3 in maxicells. The leuB-complementing activity was in a region of 1.4 kb and directed synthesis of a protein of 46,000 Da with a pI of 5.9.
通过在缺乏必需氨基酸的情况下选择大肠杆菌突变菌株进行生长，在携带谷氨酰磷酸还原酶（proA）和β-异丙基苹果酸脱氢酶（leuB）基因的流产布鲁氏菌文库中发现了克隆。这些克隆与流产布鲁氏菌DNA基因组消化产物中的独特片段杂交。发现proA互补DNA位于1.3 kb的区域，该区域在大细胞中指导合成一种48,000 Da、pI为6.3的蛋白质。leuB互补活性位于1.4 kb的区域，指导合成一种46,000 Da、pI为5.9的蛋白质。
