中国癫痫患者血清和唾液中游离苯妥英的监测

Free phenytoin monitoring in serum and saliva of epileptic patients in China.

作者信息

Cai W M, Zhu G Z, Chen G

机构信息

Department of Clinical Pharmacology, Jinling Hospital, Nanjing, People's Republic of China.

出版信息

Ther Drug Monit. 1993 Feb;15(1):31-4. doi: 10.1097/00007691-199302000-00005.

DOI:10.1097/00007691-199302000-00005

PMID:8451777

Abstract

Twenty-three serum samples and 13 saliva samples from 14 Chinese epileptic patients treated with phenytoin (DPH) were obtained to study the correlations between total, free serum, and saliva DPH concentrations. The binding of DPH to serum protein was assessed by equilibrium dialysis, and total and free DPH concentrations were analyzed with a domestic fluorescence polarization immunoassay reagent by the TDx system. A strong correlation existed between the total and free concentrations (r = 0.90, n = 20, p < 0.001). The mean value for the DPH free fraction was 11.13 +/- 3.65%. The mean value for the saliva fraction was 9.40 +/- 2.60%. This is the first attempt to monitor free DPH concentrations in serum in Chinese patients.

摘要

采集了14例接受苯妥英（DPH）治疗的中国癫痫患者的23份血清样本和13份唾液样本，以研究血清总DPH浓度、游离血清DPH浓度和唾液DPH浓度之间的相关性。通过平衡透析评估DPH与血清蛋白的结合情况，并使用国产荧光偏振免疫分析试剂通过TDx系统分析血清总DPH浓度和游离DPH浓度。总浓度和游离浓度之间存在强相关性（r = 0.90，n = 20，p < 0.001）。DPH游离分数的平均值为11.13±3.65%。唾液分数的平均值为9.40±2.60%。这是首次在中国患者中监测血清游离DPH浓度的尝试。