Cook C E, Amerson E, Poole W K, Lesser P, O'Tuama L
Clin Pharmacol Ther. 1975 Dec;18(6):742-7. doi: 10.1002/cpt1975186742.
Saliva and plasma levels of phenytoin (DPH) and phenobarbital (PB) in a series of epileptic patients were compared by means of a radioimmunoassat (RIA) that required only 10 mul of saliva or plasma. There was an excellent linear relation (r = 0.98) between the logarithms of the concentrations of DPH in the two fluids. The ratio saliva/plasma was remarkably constant at 0.10 and was unaffected by varying levels of PB. The ratio was close to the fraction of DPH reported unbound in plasma at 37 degrees. PB plasma and saliva levels were also closely related (r = 0.98 for logarithm of plasma and saliva levels). This relation was nonlinear [plasma ocncentration = 4.43 X (salivary concentration)0.86], but could be approximated by the ratio plasma/saliva = 3.4. The simplicity of sample collection and the sensitivity of the RIA procedure suggest that clinical monitoring of these anticonvulsant levels may be carried out by RIA on saliva samples.
通过一种仅需10微升唾液或血浆的放射免疫分析（RIA）方法，对一系列癫痫患者的唾液和血浆中苯妥英（DPH）和苯巴比妥（PB）的水平进行了比较。两种液体中DPH浓度的对数之间存在极佳的线性关系（r = 0.98）。唾液/血浆的比值非常恒定，为0.10，且不受PB不同水平的影响。该比值接近37摄氏度时血浆中未结合的DPH比例。PB的血浆和唾液水平也密切相关（血浆和唾液水平对数的r = 0.98）。这种关系是非线性的[血浆浓度 = 4.43×（唾液浓度）^0.86]，但可用血浆/唾液比值 = 3.4来近似。样本采集的简便性和RIA程序的敏感性表明，这些抗惊厥药水平的临床监测可通过对唾液样本进行RIA来进行。
