淋巴因子激活的杀伤细胞对头颈部肿瘤细胞集落生长的抑制作用。

Inhibition of head and neck tumor cell colony growth by lymphokine activated killer cells.

作者信息

Kumazawa H, Kita J, Sai S, Tachikawa T, Hori Y, Yamazaki N, Yamashita T, Kumazawa T, Kawamoto K, Koldovsky P

机构信息

Department of Otolaryngology, Kansai Medical University, Osaka, Japan.

出版信息

Acta Otolaryngol Suppl. 1993;500:138-41. doi: 10.3109/00016489309126198.

DOI:10.3109/00016489309126198

PMID:8452012

Abstract

The antiproliferative effect of lymphokine activated killer (LAK) cells on head and neck tumor cells has not previously been elucidated. We studied the inhibitory effects of recombinant interleukin-2 activated lymphocytes on tumor colony formation in semisolid agar, using head and neck tumor cells prepared from established tumor cell lines (K562, HT29, HLaC78) and xenografted head and neck squamous cell carcinomas on nude mice (XKN, XLL, XFL, XKF). LAK cells demonstrated a significant inhibitory effect on colony formation. The effects of LAK cells on cultured tumor cell lines were evaluated in a dose dependent manner using effector: target ratios. In addition, the colony formation of tumor cells derived from xenografted nude mouse was inhibited by LAK cells. These results suggest that LAK cells generate an antiproliferative effect on head and neck tumor cells.

摘要

淋巴因子激活的杀伤（LAK）细胞对头颈部肿瘤细胞的抗增殖作用此前尚未阐明。我们使用从已建立的肿瘤细胞系（K562、HT29、HLaC78）制备的头颈部肿瘤细胞以及裸鼠体内异种移植的头颈部鳞状细胞癌（XKN、XLL、XFL、XKF），研究了重组白细胞介素-2激活的淋巴细胞对半固体琼脂中肿瘤集落形成的抑制作用。LAK细胞对集落形成显示出显著的抑制作用。使用效应细胞与靶细胞比例，以剂量依赖方式评估LAK细胞对培养的肿瘤细胞系的作用。此外，LAK细胞抑制了源自异种移植裸鼠的肿瘤细胞的集落形成。这些结果表明LAK细胞对头颈部肿瘤细胞产生抗增殖作用。