Sonko B J, Murgatroyd P R, Goldberg G R, Coward W A, Ceesay S M, Prentice A M
Medical Research Council, Dunn Clinical Nutrition Centre, Cambridge, UK.
Acta Physiol Scand. 1993 Jan;147(1):99-108. doi: 10.1111/j.1748-1716.1993.tb09477.x.
A non-invasive method for studying the dynamics of post-exercise carbohydrate storage by means of whole-body calorimetry and 13CO2 breath tests is described. Seven untrained glycogen-depleted subjects were offered naturally 13C-labelled high carbohydrate meals (97% by energy) at 30 min intervals for 5 h and asked to consume as much as possible. Mean intake averaged 757 +/- 211 (SD) g. Exogenous carbohydrate oxidation over 16 h calculated from gas exchange and isotope ratio measurements averaged 161 +/- 45 g, and endogenous carbohydrate oxidation averaged 31 +/- 25 g. Net carbohydrate storage, calculated as the difference between amount ingested and oxidized, was 563 g which was more than twice the measured hepatic and muscle carbohydrate oxidized during the depletion phase. After correction for body size the major determinant of glycogen storage was the amount of carbohydrate consumed (r = 0.97, P < 0.001) which in turn was determined by each subject's dietary tolerance. Post-repletion exercises (12 h after last meal) were used to remobilize freshly stored glycogen. 13CO2 enrichments indicated that a substantial part of the new glycogen was derived from the exogenous carbohydrate provided by the repletion meals.
本文描述了一种通过全身量热法和13CO2呼气试验研究运动后碳水化合物储存动态的非侵入性方法。七名未经训练且糖原耗尽的受试者每隔30分钟自然摄入13C标记的高碳水化合物餐（能量占比97%），持续5小时，并被要求尽可能多吃。平均摄入量为757±211（标准差）克。根据气体交换和同位素比率测量计算得出的16小时外源性碳水化合物氧化平均为161±45克，内源性碳水化合物氧化平均为31±25克。净碳水化合物储存量，即摄入与氧化量之差，为563克，是糖原耗尽阶段肝脏和肌肉中氧化的碳水化合物量的两倍多。校正身体大小后，糖原储存的主要决定因素是碳水化合物摄入量（r = 0.97，P < 0.001），而碳水化合物摄入量又取决于每个受试者的饮食耐受性。补充后运动（最后一餐12小时后）用于重新调动新储存的糖原。13CO2富集表明，新糖原的很大一部分来自补充餐提供的外源性碳水化合物。
