Taulli T A, Hill J T, Pounds G W
J Chromatogr Sci. 1977 Mar-Apr;15(3-4):111-8. doi: 10.1093/chromsci/15.3-4.111.
A reversed-phase high performance liquid chromatographic (HPLC) procedure for the analyses of 3,4,4'-trichlorocarbanilide (TCC) and its free and/or conjugated metabolic products in plasma/serum in described. A rapid, effective clean-up procedure, prior to chromatographic evaluation, involves a single-step combined protein removal and THF extraction. Detection of the TCC moiety after HPLC separation is by UV absorption at 265 nm; quantitation by peak height measurement. A detection limit of 10 ppb of TCC and/or metabolities has been demonstrated for this method. Verification of this method was by radiotracer counting of the appropriate HPLC peaks from plasma of animals adminstered 14C-TCC/TCC. The utility of this method was demonstrated in both animal pharmacological and toxicological studies and in human bathing studies.
本文描述了一种反相高效液相色谱（HPLC）方法，用于分析血浆/血清中的3,4,4'-三氯碳酰替苯胺（TCC）及其游离和/或结合代谢产物。在进行色谱评估之前，一种快速、有效的净化程序包括一步去除蛋白质并结合四氢呋喃萃取。HPLC分离后，通过在265nm处的紫外吸收检测TCC部分；通过峰高测量进行定量。该方法已证明对TCC和/或代谢物的检测限为10ppb。通过对给予14C-TCC/TCC的动物血浆中适当的HPLC峰进行放射性示踪计数来验证该方法。该方法在动物药理学和毒理学研究以及人体沐浴研究中均得到了验证。
