从可透析白细胞提取物中分离β2-微球蛋白与转移因子。
Separation of beta2-microglobulin from transfer factor in dialyzable leukocyte extracts.
作者信息
Nékám K, Kelemen G S, Varró R, Fekete B, Láng I, Kalmár L, Gergely P, Petrányi G
出版信息
Allerg Immunol (Leipz). 1978;24(4):254-8.
Dialyzable leukocyte extracts prepared according to the original method of LAWRENCE contain 4 X 10(4) molecules of beta2-microglobulin per lymphocyte equivalent. The negative skin test converting biological activity (transfer factor) is separable by means of Sephadex G-25 gel chromatography from the beta2-microglobulin component of the extracts. This finding does not support the hypothesis of SHIFRINE and SCIBIENSKI on beta2-microglobulin being the nonspecific anchor of specific transfer factor to nonsensitized lymphocytes.
按照劳伦斯的原始方法制备的可透析白细胞提取物，每淋巴细胞当量含有4×10⁴个β2-微球蛋白分子。阴性皮肤试验转化生物活性（转移因子）可通过Sephadex G-25凝胶色谱法从提取物的β2-微球蛋白组分中分离出来。这一发现不支持希夫林和斯奇宾斯基关于β2-微球蛋白是特异性转移因子与未致敏淋巴细胞的非特异性锚定物的假说。
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