Liou R F, Chang W C, Chu S T, Chen Y H
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China.
Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):591-4. doi: 10.1042/bj2900591.
The action of Taiwan cobra (Naja naja atra) venom cardiotoxin on rabbit platelets at 37 degrees C was characterized by observing cytoskeletal alterations and cell lysis. At a concentration of 21.4 microM the toxin produced no cell lysis within 30 s, and less than 5% of the total lactate dehydrogenase activity of intact cells was detected in the suspending medium after the interaction had proceeded for 3 min. The extent of cell lysis was proportional to toxin concentration and interaction time. Before cell lysis, the toxin caused rapid incorporation of actin monomers into cross-linked actin filaments. The actin incorporation could be inhibited by either the presence of cytochalasin B or increased CaCl2 concentration in the suspending medium. However, addition of indomethacin did not influence the toxin-induced cytoskeletal change.
通过观察细胞骨架变化和细胞裂解，对台湾眼镜蛇（眼镜蛇）毒液心脏毒素在37℃下对兔血小板的作用进行了表征。在21.4微摩尔的浓度下，毒素在30秒内未产生细胞裂解，相互作用进行3分钟后，在悬浮培养基中检测到的完整细胞总乳酸脱氢酶活性不到5%。细胞裂解程度与毒素浓度和相互作用时间成正比。在细胞裂解之前，毒素导致肌动蛋白单体迅速掺入交联肌动蛋白丝中。细胞松弛素B的存在或悬浮培养基中氯化钙浓度的增加均可抑制肌动蛋白掺入。然而，添加吲哚美辛并不影响毒素诱导的细胞骨架变化。
