Van der Veer E, Van der Weide D, Heijmans H S, Hoekstra D
Department of Pediatrics, University of Groningen, The Netherlands.
Biochim Biophys Acta. 1993 Mar 14;1146(2):294-300. doi: 10.1016/0005-2736(93)90368-a.
Fluorescently labelled ether phospholipid (1-O-alkyl/alkenyl-2-acyl- glycerophosphocholine) readily internalizes at low temperatures (2 degrees C) after insertion into the plasma membrane of cultured fibroblasts. This fate differs markedly from that of its diacyl phospholipid analogue, which remains associated with the plasma membrane under similar conditions. Analysis by thin-layer chromatography reveals that the translocation involves transfer of the intact ether phosphatidylcholine molecules. Relative to control cells, a 2-fold increase of ether phosphatidylcholine uptake was noted when plasmalogen deficient fibroblasts were used. Back-exchange experiments demonstrate that more than 60% of the cell-associated ether lipid is translocated within the cells, irrespective of the cell strain that was used. The potential mechanism by which the translocation process is accomplished is discussed.
荧光标记的醚磷脂（1-O-烷基/烯基-2-酰基甘油磷酸胆碱）在插入培养的成纤维细胞质膜后，于低温（2摄氏度）下很容易内化。这种命运与其二酰基磷脂类似物明显不同，后者在类似条件下仍与质膜结合。薄层色谱分析表明，转位涉及完整醚磷脂酰胆碱分子的转移。相对于对照细胞，当使用缺乏缩醛磷脂的成纤维细胞时，醚磷脂酰胆碱的摄取增加了2倍。反向交换实验表明，无论使用何种细胞系，超过60%与细胞相关的醚脂都会在细胞内转位。文中讨论了转位过程完成的潜在机制。
