Band A M, Jones P M, Howell S L
Biomedical Sciences Division, King's College London, UK.
Biochim Biophys Acta. 1993 Mar 10;1176(1-2):64-8. doi: 10.1016/0167-4889(93)90178-r.
The stimulation of rat pancreatic islets by glucose leads both to the secretion of insulin, and the production of arachidonic acid (AA). We have previously shown that exogenous AA can stimulate insulin secretion and that this secretion was not dependent upon extracellular Ca2+ nor upon the activation of protein kinase C. We have now demonstrated that AA-induced insulin secretion was a saturable and reversible process. AA-stimulated insulin secretion was slow in onset from intact islets but immediate from electrically permeabilized islets. In permeabilized islets AA-induced insulin secretion was not dependent on changes in intracellular Ca2+ or ATP and was not inhibited by noradrenaline. These results suggest that AA affects insulin secretion at a late stage in the exocytotic process.
葡萄糖对大鼠胰岛的刺激会导致胰岛素分泌以及花生四烯酸（AA）的产生。我们之前已经表明，外源性AA能够刺激胰岛素分泌，并且这种分泌不依赖于细胞外Ca2+，也不依赖于蛋白激酶C的激活。我们现在已经证明，AA诱导的胰岛素分泌是一个可饱和且可逆的过程。AA刺激的胰岛素分泌从完整胰岛开始时较慢，但从电通透化胰岛开始时则是即时的。在通透化胰岛中，AA诱导的胰岛素分泌不依赖于细胞内Ca2+或ATP的变化，也不受去甲肾上腺素的抑制。这些结果表明，AA在胞吐过程的后期影响胰岛素分泌。
