Ebert E C
Department of Medicine, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick 08903.
Cell Immunol. 1993 Apr 1;147(2):331-40. doi: 10.1006/cimm.1993.1073.
Human intraepithelial lymphocytes (IEL) are predominantly CD45RO+ (memory) CD8+ T lymphocytes located between intestinal epithelial cells. This study determines whether IEL share other characteristics with circulating CD45RO+ lymphocytes. Memory cells are large and have an upregulated expression of adhesion molecules. In contrast, the majority of IEL are the same size as peripheral blood (PB) T cells. In addition, IEL do not have an increased density of adhesion molecules, although a larger percentage express CD54 and CD58 compared to PBL. Allo-CTL activity, demonstrated by CD45RO+ PBL, could not be shown using either IEL or lamina propria lymphocytes (LPL) after a 5-day culture with alloantigen. However, after a 2.5-week culture with allogeneic PBL and interleukin-2 (IL-2), both IEL and LPL were capable of this cytotoxic function. The addition of IL-4 or IL-6 did not alter CTL activity by mucosal lymphocytes. Alloantigen-stimulated IEL, LPL, and PB CD8+ T cell lines were propagated for up to 16 weeks. All lines demonstrated alloantigen-specific proliferation. The PB CD8+ T lymphocytes maintained their phenotype and allo-CTL activity. In contrast, the CD4+ subset in the IEL and LPL became the predominant lymphocyte type and demonstrated potent lytic activity that was not alloantigen specific. This study shows that (1) IEL do not have increased density of adhesion molecules, (2) mucosal lymphocytes demonstrate allo-CTL activity, (3) CD8+ T cells in the mucosa cannot be perpetuated long-term by alloantigen and IL-2, and (4) mucosal CD4+ T cell lines demonstrate marked alloantigen-nonspecific cytotoxicity. The only characteristic shared by IEL and circulating memory cells is allo-CTL activity.
人上皮内淋巴细胞（IEL）主要是位于肠上皮细胞之间的CD45RO +（记忆性）CD8 + T淋巴细胞。本研究确定IEL是否与循环CD45RO +淋巴细胞具有其他共同特征。记忆细胞体积较大，黏附分子表达上调。相比之下，大多数IEL与外周血（PB）T细胞大小相同。此外，IEL的黏附分子密度并未增加，尽管与外周血淋巴细胞（PBL）相比，表达CD54和CD58的IEL百分比更高。用同种异体抗原培养5天后，CD45RO + PBL所表现出的同种异体细胞毒性T淋巴细胞（allo - CTL）活性，在IEL或固有层淋巴细胞（LPL）中均未表现出来。然而，在用同种异体PBL和白细胞介素-2（IL - 2）进行2.5周培养后，IEL和LPL均具备这种细胞毒性功能。添加IL - 4或IL - 6不会改变黏膜淋巴细胞的CTL活性。用同种异体抗原刺激IEL、LPL和PB CD8 + T细胞系传代培养长达16周。所有细胞系均表现出同种异体抗原特异性增殖。PB CD8 + T淋巴细胞保持其表型和allo - CTL活性。相比之下，IEL和LPL中的CD4 +亚群成为主要淋巴细胞类型，并表现出非同种异体抗原特异性的强大溶解活性。本研究表明：（1）IEL的黏附分子密度没有增加；（2）黏膜淋巴细胞表现出allo - CTL活性；（3）黏膜中的CD8 + T细胞不能通过同种异体抗原和IL - 2长期维持；（4）黏膜CD4 + T细胞系表现出明显的非同种异体抗原特异性细胞毒性。IEL与循环记忆细胞唯一的共同特征是allo - CTL活性。
