Kaspar P, Dvorák M, Bartůnĕk P
Institute of Molecular Genetics, Czech Academy of Sciences, Prague, Czech Republic.
FEBS Lett. 1993 Mar 15;319(1-2):159-62. doi: 10.1016/0014-5793(93)80058-3.
We identified a CpG island at the 5' end of murine leukemia inhibitory factor gene (LIF). The CpG island is 0.6 kb long and covers most of the first exon and first intron. The region is non-methylated, its G+C content is 65% and relative frequency of CpG dinucleotide is 0.7. The block of 150 nucleotides, which is 72% conserved between murine, human, ovine and porcine genes, is a part of the CpG island. Two DNA fragments from this CpG island interact with nuclear proteins from NIH 3T3 cells. One fragment partially covers the block of conserved nucleotides. Human, ovine and porcine LIF genes also contain G+C- and CpG-rich sequences in the corresponding region.
我们在小鼠白血病抑制因子基因（LIF）的5'端鉴定出一个CpG岛。该CpG岛长0.6 kb，覆盖了大部分第一外显子和第一内含子。该区域未甲基化，其G+C含量为65%，CpG二核苷酸的相对频率为0.7。在小鼠、人类、绵羊和猪基因之间72%保守的150个核苷酸片段是该CpG岛的一部分。来自该CpG岛的两个DNA片段与NIH 3T3细胞的核蛋白相互作用。一个片段部分覆盖保守核苷酸片段。人类、绵羊和猪的LIF基因在相应区域也含有富含G+C和CpG的序列。
