Homer-Vanniasinkam S, Hardy S C, Gough M J
Vascular Surgical Unit, General Infirmary, Leeds, U.K.
Eur J Vasc Surg. 1993 Jan;7(1):41-5. doi: 10.1016/s0950-821x(05)80542-0.
The role of a novel inhibitor of lipid peroxidation (U74500A) in modifying post-ischaemic changes in skeletal muscle blood flow, viability and oedema has been investigated in a rat model of 6 h unilateral hindlimb ischaemia followed by 4 h reperfusion. During reperfusion, gastrocnemius muscle blood flow (GMBF) was measured (radiolabelled microspheres) at 10, 120 and 240 min and a perfusion index (PI) calculated between the reperfused and contralateral normal limb. Muscle viability (GMV--nitroblue tetrazolium) and oedema (wet:dry weight ratio, reperfused: contralateral limb) were assessed after 4 h reperfusion. GMBF in untreated controls, compared to normal animals, demonstrated a triphasic pattern of low reflow at 10 min [PI 0.08 (0.01-0.13) vs. 1.05 (0.68-1.18), p < 0.01], relative reperfusion at 120 min [PI 0.29 (0.09-0.59) vs. 0.97 (0.79-1.13), p < 0.05] and reperfusion injury at 240 min [PI 0.05 (0.01-0.14) vs. 1.01 (0.73-1.16), p < 0.01] with a median GMV at 240 min of 52.9% (33.3-61.4), p < 0.01 vs. normals and 6 h ischaemia alone (GMV 100% in all limbs) and an oedema index of 1.23 (1.09-1.37 p < 0.01 vs. normal. In contrast, rats receiving U74500A (2 mg/kg i.v. infusion commencing 30 min prior to revascularisation) exhibited enhanced GMBF throughout reperfusion [PI 10 min: 3.26 (2.56-3.63); 120 min: 2.03 (1.73-2.25); 240 min: 2.13 (1.75-2.44), p < 0.01 vs. controls and normals] with complete muscle salvage [GMV 100% in all reperfused muscles, p < 0.01 vs. controls, not significant (NS) vs. normals and 6 h ischaemia].(ABSTRACT TRUNCATED AT 250 WORDS)
在大鼠单侧后肢缺血6小时再灌注4小时的模型中，研究了一种新型脂质过氧化抑制剂（U74500A）对骨骼肌血流、活力和水肿的缺血后变化的影响。再灌注期间，在10、120和240分钟时测量腓肠肌血流量（GMBF）（放射性标记微球），并计算再灌注肢体与对侧正常肢体之间的灌注指数（PI）。再灌注4小时后评估肌肉活力（GMV-硝基蓝四氮唑）和水肿（湿重：干重比，再灌注肢体：对侧肢体）。与正常动物相比，未治疗对照组的GMBF呈现三相模式：10分钟时低再流[PI 0.08（0.01 - 0.13）对1.05（0.68 - 1.18），p < 0.01]，120分钟时相对再灌注[PI 0.29（0.09 - 0.59）对0.97（0.79 - 1.13），p < 0.05]，240分钟时再灌注损伤[PI 0.05（0.01 - 0.14）对1.01（0.73 - 1.16），p < 0.01]，240分钟时GMV中位数为52.9%（33.3 - 61.4），与正常组和仅缺血6小时组相比差异有统计学意义（p < 0.01）（所有肢体GMV为100%），水肿指数为1.23（1.09 - 1.37，与正常组相比p < 0.01）。相比之下，接受U74500A（血管再通前30分钟开始静脉输注2mg/kg）的大鼠在整个再灌注过程中GMBF增强[PI 10分钟：3.26（2.56 - 3.63）；120分钟：2.03（1.73 - 2.25）；240分钟：2.13（1.75 - 2.44），与对照组和正常组相比p < 0.01]，肌肉完全挽救[所有再灌注肌肉GMV为100%，与对照组相比p < 0.01，与正常组和缺血6小时组相比无统计学意义（NS）]。（摘要截断于250字）
