Hunter K E, Sporn M B, Davies A M
Department of Anatomy, St. George's Hospital Medical School, London.
Glia. 1993 Mar;7(3):203-11. doi: 10.1002/glia.440070303.
We have studied the influence of three members of the transforming growth factor-beta (TGF-beta) family of multifunctional growth factors on the proliferation of cultured astrocytes isolated from newborn mouse cerebral cortex. Although TGF-beta s 1, 2, and 3 cause only a small reduction in the low level of astrocyte proliferation occurring in chemically defined medium, they each inhibit the effects of five astrocyte mitogens (bFGF, EGF, PDGF, IL-1 alpha, and IL-2). Inhibition is observed when astrocytes are exposed to mitogen and TGF-beta at the same time and when they are exposed to TGF-beta prior to, and separately from, mitogen. This latter effect appears to be due to the binding of TGF-beta s to astrocyte-secreted extracellular matrix. These findings raise the possibility that TGF-beta may co-operate with other growth factors to control astrocyte proliferation in vivo.
我们研究了多功能生长因子转化生长因子-β（TGF-β）家族的三个成员对从新生小鼠大脑皮层分离的培养星形胶质细胞增殖的影响。尽管TGF-β 1、2和3仅使在化学成分确定的培养基中发生的低水平星形胶质细胞增殖略有减少，但它们各自都能抑制五种星形胶质细胞有丝分裂原（bFGF、EGF、PDGF、IL-1α和IL-2）的作用。当星形胶质细胞同时暴露于有丝分裂原和TGF-β时，以及当它们在暴露于有丝分裂原之前且与之分开暴露于TGF-β时，均观察到抑制作用。后一种效应似乎是由于TGF-β与星形胶质细胞分泌的细胞外基质结合所致。这些发现增加了TGF-β可能与其他生长因子协同控制体内星形胶质细胞增殖的可能性。
