Nadir S, Saacke R G, Bame J, Mullins J, Degelos S
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg 24061.
J Anim Sci. 1993 Jan;71(1):199-204. doi: 10.2527/1993.711199x.
This experiment was conducted to determine whether use of fresh or frozen semen at either 20 x 10(6) (low) or 100 x 10(6) (high) sperm per dose affects the number of accessory sperm and fertilization status/embryo quality as determined from ova/embryos recovered nonsurgically 6 d after insemination. Ejaculates of four bulls were split and prepared for use as fresh or frozen semen at either the high or low dose. From 129 inseminations to normally cycling cows, 98 ova/embryos were recovered. To reduce male effects, ova/embryos used were randomly balanced across treatments, by ejaculate within bull for evaluation of frozen vs fresh semen (n = 80) and by bull for evaluation of high vs low dosage treatments (n = 76). Distribution of accessory sperm was highly skewed downward; thus, median values were more meaningful than means. Freezing semen had no significant effect on fertility status/embryo quality or number of accessory sperm at either dosage. Increasing dosage improved the number of accessory sperm per ovum or embryo (median value) and fertility status/embryo quality (P < .05). Mean +/- SD and median values for accessory sperm were 37.8 +/- 38.3 and 27.5; 28.9 +/- 62.8 and 3.0 for the high and low dose, respectively. Percentage of unfertilized ova, degenerate embryos, and embryos classified poor to fair and good to excellent were 3, 5, 24, 68, and 21, 16, 18, 45, for the high and low dose, respectively. We conclude that number of accessory sperm and fertility status/embryo quality respond favorably to increased dosage of semen and that freezing semen in this study was not detrimental to these parameters.
本实验旨在确定每剂量使用20×10⁶（低剂量）或100×10⁶（高剂量）的新鲜或冷冻精液，是否会影响授精6天后通过非手术回收的卵子/胚胎所确定的附属精子数量以及受精状态/胚胎质量。将四头公牛的射精精液分开，制备成高剂量或低剂量的新鲜或冷冻精液。对正常发情的母牛进行了129次授精，回收了98个卵子/胚胎。为减少雄性效应，所使用的卵子/胚胎在各处理间随机平衡，在公牛内按射精区分用于评估冷冻精液与新鲜精液（n = 80），按公牛区分用于评估高剂量与低剂量处理（n = 76）。附属精子的分布严重向下偏态；因此，中位数比均值更有意义。冷冻精液对两种剂量下的生育状态/胚胎质量或附属精子数量均无显著影响。增加剂量可提高每个卵子或胚胎的附属精子数量（中位数）以及生育状态/胚胎质量（P < 0.05）。高剂量和低剂量附属精子的均值±标准差及中位数分别为37.8±38.3和27.5；28.9±62.8和3.0。高剂量和低剂量未受精卵子、退化胚胎以及分类为差至中等和良好至优秀的胚胎百分比分别为3%、5%、24%、68%和21%、16%、18%、45%。我们得出结论，附属精子数量和生育状态/胚胎质量对精液剂量增加反应良好，且本研究中冷冻精液对这些参数无害。
