Aspinall G O, McDonald A G, Pang H, Kurjanczyk L A, Penner J L
Department of Chemistry, York University, Toronto, Ontario, Canada.
J Biol Chem. 1993 Mar 25;268(9):6263-8.
Lipopolysaccharide from phenol-water extraction of cells of Campylobacter coli serotype O:30 was separated as a water-insoluble gel of low M(r) from a water-soluble antigenic polysaccharide of high M(r). Acetic acid hydrolysis of the ketosidic linkages to lipid A in the lipopolysaccharide furnished a core oligosaccharide. Structural investigations were performed using 1H and 13C NMR, fast atom bombardment-mass spectrometry of permethylated derivatives, and methylation linkage analysis on the core oligosaccharide and the products of two successive Smith degradations. It is concluded that the highly branched 3-deoxy-D-manno-octulosonic acid-terminated oligosaccharide chains carried at the nonreducing end disaccharide units of beta-D-Qui3NAc-(1-->2)-beta-D-Qui3NAc (where Qui3NAc represents 3-acylamino-3,6-dideoxy-D-glucose), in which N-acyl residues were either both (R)-3-hydroxybutanoyl or both 3-hydroxy-2,3-dimethyl-5-oxoprolyl. The demonstration of these unusual features provides further evidence for a wide variety of structures within the core oligosaccharide region of lipopolysaccharides from Campylobacter sp.
从空肠弯曲菌O:30血清型细胞的酚水提取物中获得的脂多糖，是从一种高分子量的水溶性抗原多糖中分离出来的低分子量水不溶性凝胶。脂多糖中与脂质A相连的酮糖苷键经乙酸水解后得到一种核心寡糖。利用1H和13C NMR、全甲基化衍生物的快原子轰击质谱以及对核心寡糖和两次连续Smith降解产物的甲基化连接分析进行了结构研究。得出的结论是，高度分支的以3-脱氧-D-甘露-辛酮糖酸结尾的寡糖链在非还原端带有β-D-Qui3NAc-(1→2)-β-D-Qui3NAc的二糖单元（其中Qui3NAc代表3-酰氨基-3,6-二脱氧-D-葡萄糖），其中N-酰基残基要么都是(R)-3-羟基丁酰基，要么都是3-羟基-2,3-二甲基-5-氧代脯氨酰基。这些不寻常特征的证明为弯曲菌属脂多糖核心寡糖区域内多种多样的结构提供了进一步的证据。
