Arvinte T, Cudd A, Drake A F
Ciba-Geigy Pharmaceuticals, Horsham, United Kingdom.
J Biol Chem. 1993 Mar 25;268(9):6415-22.
Turbidity measurements of the kinetics of human calcitonin (hCT) fibrillation showed a linear dependence of the logarithm of fibrillation time (the time the sample is not fibrillated) and the logarithm of hCT concentration. This ln/ln plot linearity and electron microscope observations of fibrils indicate that the fibrillation process can be explained by the double nucleation mechanism that was proposed for the gelation of sickle cell hemoglobin (Ferrone, F. A., Hofrichter, J., Sunshine, H. R., and Eaton, W. A. (1980) Biophys. J. 32, 361-380). Circular dichroism, fluorescence, and infrared spectroscopy studies of fibrils showed that hCT molecules have alpha-helical and beta-sheet secondary structure components. A model for the structure of hCT molecules in fibrils is proposed.
对人降钙素（hCT）纤维化动力学的浊度测量表明，纤维化时间（样品未发生纤维化的时间）的对数与hCT浓度的对数呈线性关系。这种ln/ln图的线性以及对纤维的电子显微镜观察表明，纤维化过程可以用为镰状细胞血红蛋白凝胶化所提出的双核化机制来解释（费罗内，F.A.，霍夫里希特，J.，桑夏恩，H.R.，和伊顿，W.A.（1980年）《生物物理学杂志》32卷，361 - 380页）。对纤维的圆二色性、荧光和红外光谱研究表明，hCT分子具有α - 螺旋和β - 折叠二级结构成分。提出了纤维中hCT分子结构的模型。
